Наукові журнали та збірники видань
Постійне посилання на розділhttps://dglib.nubip.edu.ua/handle/123456789/5
Переглянути
2 результатів
Результати пошуку
Документ The influence of allogeneic bone marrow derived mesenchymal stem cells on indicators of functional state of immune organs in mice C57BL/6(ВЦ НУБіП України, 2018) Kladnytska, L.V.; Mazurkevych, A.I.; Velychko, S.V.; Kharkevych, Y.O.; Shelest, D.; Velychko, V.S.The studies were conducted on 2-3-months-old males of mice weighing 20-24 g. Оur work was to study the functional state of the organs of the immune system of C57Bl/6 mice after introduction of allogeneic MSCs of bone marrow origin. Obtaining and cultivating of MSCs were carried out in a sterile laminar box with compliance of conditions of asepsis and antiseptics. C57Bl/6 mice bone marrow aspirate cultured in a CO2 incubator at 37 оC and 5 % CO2 in DMEM with 10-15 % of fetal bovine serum, 1 % of antibiotic-antimycotic solution (Sigma-Aldrich, USA). The following groups of animals were formed: 1 group – intact (control group); 2 group - animals, to whom 0.5 ml of 0.9 % NaCl solution (placebo) were injected into the caudal vein; 3 group – animals, to whom 104 of allogeneic MSCs in 0.5 ml of phosphate buffer solution were injected into the caudal vein. The weight index, cellularity of thymus and spleen in C57Bl/6 mice investigated after the introduction of MSCs. The administration of allogenic mesenchymal stem cells of the bone marrow origin affects on the central and peripheral organs of the immune system. Administration of allogenic mesenchymal stem cells cause a significant increase in the content of lymphoid cells in the thymus at 7 and 18 days by 72 and 39 %, respectively (p < 0.01, p < 0.05) compared to the control. Administration of allogenic mesenchymal stem cells cause a significant increase in the weight index of the spleen and its cellularity at the 7 and 18 days of the immune response by 26 and 17 %, respectively (p < 0.01, p <0.05) compared to the control. the weight index of the spleen and it cellularity a significant increase at the 7 and 18 days of the immune response by 26 and 17 %, respectively (p < 0.05) compared to the control after administration of allogenic mesenchymal stem cells.Документ Features of the thymic morphology in hens and ducks(ВЦ НУБіП України, 2018) Dyshlyuk, N.V.; Parkhomenko, A.V.The features of the thymic morphology in hens and ducks were studied. Samples were obtained from 3 adult birds of each species. They were studied by light microscopy using classical methods of morphological research. The tissue samples were fixed in 10% neutral buffered formalin and embedded in paraffin block and finally the sections were cut at 5-10 μm thickness using sliding microtome and stained with hematoxylin and eosin, according to Van Gizon and Weigert and impregnated with silver nitrate according to Kelemen. It was established that the thymus of birds was represented by isolated cervical parts, which, in the form of strands, were located under the skin on the lateral surfaces of the neck, along the trachea, along the course of the neurovascular bundle, and consisted of individual lobes. In hens 5 to 8 lobes were included in each thymus and extended from the 3-cervical vertebra to the start of the thoraco-abdominal cavity. Thymus of ducks consisted of 3-4 lobes and was located in the posterior third of the neck. Its cranial end was at the level of 10-11 cervical vertebrae, and caudal end reached 12 cervical vertebra. Individual lobes of duck’s thymus had a greater absolute mass than the lobes of hen’s thymus. They were also more separated from each other. Lobes of hen’s and duck’s thymus had a variety of shapes. Among them, there were mainly oval, convex-oval, beanshaped, rounded, flat and, sometimes, sickle-shaped. Morphofunctional units of the thymus were lobules, which consisted of a cortex and a medulla. In birds the medulla occupied a larger area than the cortex. In the medulla, there were Hassall’s corpuscles, that were better developed in ducks.